Heterogeneity of delta antigen localization in hepatocyte nucleus: immunoelectron microscopic study.
نویسندگان
چکیده
منابع مشابه
Immunoelectron microscopic study for polyamines.
The polyamines (PAs) are ubiquitous polycationic metabolites in eukaryotic and prokaryotic cells and are believed to be intimately involved in the regulation of DNA, RNA, and protein biosynthesis, the exact function of which remains unclear, mainly because of a lack of knowledge of PA subcellular localization. In this study, using immunoelectron microscopy, we have demonstrated that PAs are pre...
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Penicillinase was localized in log-phase cells of Bacillus licheniformis 749/C by labeling with ferritin-anti-penicillinase immunoglobulin G conjugate. Mildly fixed homogenized cells, isolated subcellular fractions, and frozen thin sections were labeled. The label was distributed in discrete patches in the cell envelope. The patches extended from the inside part of the membrane to the outside p...
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To elucidate the IgE binding site of mugwort (Artemisia vulgaris r.) pollen, pollen grains were frozen and fixed using a cryocut. They were incubated with antibodies according to the following sequence: Sera pool of individuals who showed mugwort-RAST class 3 or 4, biotin-labeled goat anti-human IgE antibody, streptavidin-peroxidase and diaminobenzidine. Then, they were observed under electron ...
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In this chapter, postembedding immunoelectron microscopy methods for studies of cornified cell envelopes are provided. Human epidermal tissue samples are used as the material. The samples are cryo-fixed without chemical fixation, freeze-substituted at a low temperature, and embedded in Lowicryl K11M resin. For immunostaining, colloidal gold-conjugated secondary antibodies are used. Methods for ...
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We have recently reported that salivary gland cells express the lymphatic endothelial cell marker podoplanin. The present study was aimed to immunohistochemically investigate the expression of the myoepithelial cell marker α-smooth muscle actin (SMA) on podoplanin-positive cells in mouse parotid and sublingual glands, and to elucidate podoplanin localization in salivary gland myoepithelial cell...
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ژورنال
عنوان ژورنال: Kanzo
سال: 1986
ISSN: 0451-4203,1881-3593
DOI: 10.2957/kanzo.27.831